,,,,,, species reactivity pig, human, canine, mouse, bovine, Psammomys (sand rat), sheep, rat, feline, chicken application(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using enzyme pre-digested, formalin-fixed, paraffin-embedded human tonsil sections western blot: 1:200 using mouse thymus extract clone polyclonal antibody form whole antiserum mol wt antigen mol wt 20 kDa (bovine and swine) contains 15 mM sodium azide shipped in dry ice storage temp. −20°C Gene Information human. (315609) biological source rabbit conjugate unconjugated. Immunogen synthetic human CD3 peptide (residues 156-168 of the CD3-ε chain) coupled to porcine thyroglobulin. Coupling was performed by a modification of the reported method. General description CD3 is a human cell surface glycoprotein complex involved in signal transduction following binding of antigen receptor. The CD3 antigen is expressed during thymopoiesis and on mature T cells in the periphery.

Operating System Concepts 9th Edition Instructor Manual For Blueprint. Emperor Handbook Meditation New Translations. It is also present in the majority of T-CLL and T-lymphomas and in approximately 70% of T-ALL. Cytoplasmic CD3 was recently demonstrated in 95% of thymocytes and in all T-ALL including those in which surface membrane CD3 was absent. Cerebellar Purkinje cells were also reported to express the antigen.

Chessmaster 10th Edition (Video Game) Download Chessmaster 10th Edition Minimum System Requirements OS: Windows 98/ME/2000/XP CPU: Pentium 3 @ 450 MHz Processor RAM. Chessmaster 10th Edition [CD3]/Chessmaster 10th Edition [CD3].bin Chessmaster 10th Edition [CD3]/Chessmaster 10th Edition [CD3].cue 8 files, 1.82 GB total.

B lymphocytes, monocytes, granulocytes and NK cells do not express it. Rabbit polyclonal anti-CD3, T cell antibody is reactive with T cell areas in lymphoid tissues derived from various species: human, bovine, porcine, sheep, cat, dog, rat, mouse, Psammomys (sand rat), and chicken. The molecular weight of the polypeptide recognized by the antibody in bovine and swine extracts is 20 kDa. The antibody may contain residual antibodies to thyroglobulin and may exhibit minimal background staining of epithelial and muscle tissues. Anti-Human T Cell CD3 Peptide was first described by Mason et al. The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain.

It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin′s solution and embedded in paraffin. Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species. Specificity The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain. It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin′s solution and embedded in paraffin.

Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species. Application Rabbit polyclonal anti-CD3, T cell antibody may be used for identification of T lymphocytes in peripheral blood and tissues; detection and classification of T-ALL, lymphomas and immunodeficiencies and studies on detection and purification of CD3 from lymphocytes of different species. The antibody may be used in immunoblotting and to stain T cell areas in immunohistology.

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